How long after the inoculation process should one expect to witness the emergence of mycelium within the substrate? Given the myriad variables involved, including the type of mushroom species in question and the specific environmental conditions, this query becomes increasingly pertinent. For instance, how does temperature, humidity, and the quality of the substrate influence the mycelial growth rate? Are there particular signs or indicators to monitor during the incubation period that could signal the onset of mycelium development? Moreover, what role does the inoculation method play in the timeline of mycelial colonization? It is vital to comprehend not only the timeline but also the intricacies that govern this biological phenomenon. Could the absence of seen mycelium after a certain duration indicate potential issues with the inoculation or substrate? Understanding these facets can craft a clearer picture of what to anticipate following the initial inoculation.